Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Modulation of POLD1 expression in HEK293 cells. a qRT-PCR analysis of POLD1 mRNA level in HEK293 cells transfected with pcDNA3.0-POLD1, pcDNA3.0, shPOLD1 or shControl. b Western blot analysis of POLD1 protein level in HEK293 cells transfected with pcDNA3.0-POLD1, pcDNA3.0, shPOLD1 or shControl. Untransfected HEK293 cells were used as a control (Blank). n = 3, * P < 0.01 vs. the negative control (pcDNA3.0 or shControl)

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Negative Control

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Effects of altered POLD1 expression on the growth of HEK293 cells. HEK293 cells were transfected with the indicated vectors, and cell proliferation was determined by CCK-8 assay 24, 48, and 72 h later. Data shown were the mean ± SD of the ratio for the absorbance at 450 nm. n = 3, * P < 0.01 vs. shControl

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Transfection, CCK-8 Assay

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Effects of altered POLD1 expression on e cell cycle progression of HEK293 cells. a Representative FACS plots showing cell cycle distribution of HEK293 cells transfected with the indicated vectors. b Quantification of the percentage of cells in different phases. n = 3, * P < 0.01 vs. shControl

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Transfection

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Quantification of DNA synthesis rate in HEK293 cells. a HEK293 cells were transfected with the indicated vectors, 48 h after transfection EdU-positive cells were determined by flow cytometry. b Quantification of the percentage of cells positive for EdU incorporation assay shown in ( a ). n = 3, * P < 0.01 vs. shControl

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: DNA Synthesis, Transfection, Flow Cytometry

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Effects of altered POLD1 expression on DNA damage in HEK293 cells. a HEK293 cells were transfected with the indicated vectors and treated with 150 μM H 2 O 2 for 5 min in the dark. Comet assay was then performed, and images were acquired using a fluorescence microscope to show DNA fragment migration patterns. b Quantification of the tail moments from the comet assay shown in ( a ). * P < 0.01, vs. the negative control (shControl or shControl + H 2 O 2 )

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Transfection, Single Cell Gel Electrophoresis, Fluorescence, Microscopy, Migration, Negative Control